STAINS


Streptococcus pneumoniae appear as gram-positive diplococci, although some very short chains may be seen.
The cells are somewhat tapered at the ends giving the "lancet" shaped appearance.
courtesy of Dr. Susan D. Caston of the Clinical Microbiology Laboratory of the Hospital of the University of Pennsylvania.

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The common stains in Pathology Department Click here!

HAEMATOLOGY/MICROBIOLOGY/HISTOPATHOLOGY STAINS

Gram Stain Set of 500ml each:
   • GRAM-color Staining Set
Solution 1:
Crystal violet solution 500 ml
Solution 2:
LUGOL's solution 500 ml
Solution 3 and 4:
Decolourizing solution 500 ml
each Solution 5:
Safranine solution 500 ml

A. Add crystal violet stain over the fixed culture. Let stand for 10 to 60 seconds; for thinly prepared slides, it is usually acceptable to pour the stain on and off immediately. Pour off the stain and gently rinse the excess stain with a stream of water from a faucet or a plastic water bottle. Note that the objective of this step is to wash off the stain, not the fixed culture.
B. Add the iodine solution on the smear, enough to cover the fixed culture. Let stand for 10 to 60 seconds. Pour off the iodine solution and rinse the slide with running water. Shake off the excess water from the surface.
C. Add a few drops of decolorizer so the solution trickles down the slide. Rinse it off with water after 5 seconds. The exact time to stop is when the solvent is no longer colored as it flows over the slide. Further delay will cause excess decolorization in the gram-positive cells, and the purpose of staining will be defeated.
D. Counterstain with basic fuchsin solution for 40 to 60 seconds. Wash off the solution with water. Blot with bibulous paper to remove the excess water. Alternatively, the slide may shaken to remove most of the water and air-dried.


Standard Features

Historical Record of Information
Blood Bank application is designed to be a historical module that allows organizations to maintain a permanent on-line record of:
• Donors and patients
• Transfused units
• Antigens/antibodies
• Transfusions.

Donor Recruitment and Management
Donor recruitment and management features include:
• Ability to maintain blood types, antigen/antibody profiles
• On-line tracking of donor schedules and information
• Ability to evaluate criteria, check eligibility, and schedule appointments
• On-line donor recruitment phone lists and letters
• On-line, user-defined donor questionnaires to stream line the qualification process
• User-defined error checking logic allows staff to compare current information against data collected during the donor’s last appointment
• System automatically generate deferrals when donors do not meet eligibility requirements
• Ability to generate donor statistical reports.

Inventory Tracking and Utilization
Blood Bank application allows for comprehensive inventory management by tracking physical inventory as well as inventory utilization, based on pre-transfusion data. All transfusable products are tracked from the time they are entered into inventory through final disposition. Features include:
• Ability to scan units into the inventory, either by individual unit or in batches
• Automatic requisitioning for blood bank and serology tests via software integrated system capability
• Look back functionality assuring patient safety
• Comprehensive inventory management reporting tools.

Multiple Specimen Processing
Electronic worksheets and process screens can replace the worksheets used to manually enter test results for specimens and units, creating streamlined and efficient processes, ensuring accurate information and improving patient safety. Features include:
• Preview feature showing specimens and tests in batch format
• Automatic release of crossmatches using rules-based logic
• Process screens to quickly result and efficiently track unit status
• Ability to efficiently process multiple units or specimens at one time.

Patient Reports
Standard and user-defined formats are available for activity or summary reports. Features include:
• Summary and activity reports by physician, patient, or location
• Discharge and extended summaries
• Results available electronically via the Electronic Medical Record and over the Internet.

Reporting Capabilities
Extensive reporting capabilities ensure that procedures are followed and cost effectiveness is maintained. Examples of reports include:
• Outstanding Specimen Report
• Transfusion Report
• Inventory Reports
• Unit Disposition
• Overdue Warning Report
• Unverified Results Report
• Crossmatch/Transfusion Ratio Statistics by location or provider
• Utilization Reports to assist in reviewing and ensuring appropriate use of products.

 

For more information about us, e-mail to sehmedic@stremyx.com or sehomar@tm.net.my

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